Del Mar Photonics

Multiphoton imaging references

Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
J Microsc. 2002 Nov;208(Pt 2):108-15
Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H.

Biomedizinische Strukturforschung (A0602), Deutsches Krebsforschungszentrum, PO Box 101949, D-69009 Heidelberg, Germany.

Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle trackers, e.g. Alexa and Bodipy dyes, Cy2, Cy3, DAPI, Hoechst 33342, propidium iodide, FITC and rhodamine. In accordance with the two-photon excitation theory, the majority of the investigated fluorochromes did not reveal significant discrepancies between the two-photon and the one-photon emission spectra. However, a blue-shift of the absorption maxima ranging from a few nanometres up to considerably differing courses of the spectrum was found for most fluorochromes. The potential of non-linear laser scanning fluorescence microscopy is demonstrated here by visualizing multiple intracellular structures in living cells. Combined with 3D reconstruction techniques, this approach gives a deeper insight into the spatial relationships of subcellular organelles.

More references (review in progress):

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Del Mar Photonics connection:

Trestles Femtosecond Ti:Sapphire laser
Trestles Finesse Ti:Sapphire oscillator

Mavericks Femtosecond Cr:Forsterite laser (around 1250 nm)
Multiphoton Imaging with Mavericks - introductory brochure
Focus on Microscopy 2007 abstract - Cr:Forsterite crystal
Business Wire: Del Mar Photonics Announces New Mavericks (TM) Long Wavelength Femtosecond Laser for Scientific Research
and Enhanced Biological Imaging.
High Resolution Impedance and Potential Imaging using a Mavericks Cr:Forsterite Laser

Tamarack Femtosecond Er-doped fiber laser (around 1560 nm)
Buccaneer Er-doped Femtosecond OA Fiber Laser (1560nm)
Buccaneer SHG (780nm) femtosecond fiber lasers
SHG unit for femtosecond fiber lasers
Cannon Ultra-Broadband Light Source
Femtosecond Fiber Lasers from Del Mar Photonics - Er - Yb (pdf)

Negative prechirp in multiphoton microscopy

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